APRI.HMWoTau
ELISA Service
About our HMWoTau ELISA
Our highly sensitive novel ELISA detects HMWoTau species in a disease specific manner. Our ELISA (APRI.HMWoTauELISA) employs APNmAb005 mouse monoclonal antibody that recognizes HMWoTau (about 30 nm diameter), labelled by Thioflavin (Fig. 1). APRI.HMWoTauELISA detected Alzheimer’s disease (AD)-derived HMWoTau species, in a dilution dependent manner (Fig.2A) and at Braak Stage V and VI of AD tau pathology in the frontal neocortex of AD brain (phosphate buffer saline extractable fraction) (Fig. 2B). APRI.HMWoTauELISA standard curve using recombinant human tau-derived HMWoTau showed quite a good linearity with Lowest Limit of Quantitation 0.3 pg/well (Fig. 3) (all figures are adopted from FASEB J. 2024 Nov 30;38(22): e70160). APRI.HMWoTauELISA also detects the HMWoTau species derived from transgenic mice bearing human Tau and from cell cultures.
Fig 1. Target Molecule of APNmAb005
(A) AFM image and (B) dSTORM-thioflavin labelled image.
Fig.2 Dilution and Braak stage dependency
A) Lysate dilution dependency and B) Braak stage dependency
Fig 3. Standard curve of mAb005 ELISA using rhHMWoTau
(A) Standard curve, (B) CV plot, revealing LLOW of 0.3 pg/well (3 pg/mL).
Overview of Contract-based APRI.HMWoTau ELISA service
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* Only samples that do not contain infectious materials and do not violate any laws, regulations and guidance are allowed.